FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12112777
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT122411310.105964965754756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6454535.328695475859913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATG1394300.0102.171893
ACATGAC1405750.0101.436685
TGACATC1419700.0100.476328
ATGACAT1424500.0100.224817
TACATGA1418400.0100.0136954
GACATCA1426200.099.8486949
GGCTACA1466200.097.541541
CATGACA1469900.097.142856
GCTACAT1492650.095.528412
TGACAAA682500.089.712135104
AGCAGTG133400.077.305552
GTATCAA160200.064.721969
GCGACAT16600.063.155612
CTACGTG10850.063.112523
TGGCATC18350.063.032548
GGCTAGA8100.062.1923751
GGCTACG13800.058.7078171
GGCGACA19500.057.2603871
GCTACGT12150.056.8125152
GGGCTAC5950.054.9887731
Produced by FastQC (version 0.11.5)