FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_Sambamu614_SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_Sambamu614_SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22257693
Sequences flagged as poor quality0
Sequence length110
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT1986136489.23370449938365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1303640.5857031094821912No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG785550.35293415180090765No Hit
GGCGACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT591520.2657597981965157No Hit
GGCTACATGACATCAACCATATCAGCAAAGTGATACGGGTATTATTTTTG525130.23593190902579164No Hit
GGCCACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT455110.2044731230680556No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTG323190.14520372798744235No Hit
GGCTACATGACATCAACAATATCAGCAAAAGTGATACGGGTATTATTTTT276010.12400656258490042No Hit
GGCAACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT258620.11619353362453153No Hit
GGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTT258420.11610367705224436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA201.1053804E-5104.141681
GGCGACT201.1053804E-5104.141681
GCGACTG154.603942E-4104.084782
CTCCGTG201.1110127E-5104.0351943
CCATGCC252.6957423E-7104.0050355
GGCTACA21123400.0104.0021441
CGGTTAC252.6967973E-7103.99827104
CATGCGA201.1130733E-5103.99646
CGTGACT201.1130733E-5103.99646
GACAGCC154.6195227E-4103.996399
TCGCATC154.6195227E-4103.996398
CGTGACG2200.0103.996396
TGACCGC306.5756467E-9103.996398
GGCATCG2650.0103.996399
GTGACCT154.6195227E-4103.996397
GGCTCAT9650.0103.602081
GGCTAGA9000.0103.56311
GGCTACT35900.0103.56151
GCTACAT21222300.0103.5121842
GGCGACA79900.0103.489981
Produced by FastQC (version 0.11.5)