FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_Sambamu614_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_Sambamu614_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27819589
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1312980.47196240030720804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1000120.3595020760371406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298680.1073631964871947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1234150.087.373521
AGCAGTG1345300.080.074542
GCAGTGG1477100.072.801683
GGTATCA1584750.067.870848
TGGTATC1594350.067.520887
CAGTGGT1606400.066.970674
AGTGGTA1630400.066.101675
GTGGTAT1629750.066.067026
GTATCAA1682100.063.995439
CAACGCA1594200.037.6890912-13
AGAGTAC1599300.037.45347618-19
GCAGAGT1610300.037.3025816-17
ACGCAGA1643750.036.5545514-15
ATCAACG1669100.036.0756910-11
TACATGG1653000.035.8199522-23
CATGGGG971050.035.2830724-25
TGGGGTC283100.034.3195626-27
TCAACGC1575400.034.07906712-13
CAGAGTA1609350.033.3827718-19
AACGCAG1622750.033.11031314-15
Produced by FastQC (version 0.11.5)