FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_Sambamu614_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_Sambamu614_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36286096
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1729880.4767335675901866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1305560.3597962150571392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429030.11823537037437148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1631300.087.626531
AGCAGTG1791250.079.697852
GCAGTGG1966500.072.51433
GGTATCA2100400.067.792468
TGGTATC2116350.067.3994757
CAGTGGT2132850.066.908454
AGTGGTA2164300.066.028485
GTGGTAT2163300.065.967966
GTATCAA2222650.064.077789
CAACGCA2104900.037.75900312-13
AGAGTAC2127150.037.3017118-19
GCAGAGT2139400.037.2290916-17
ACGCAGA2173500.036.62347414-15
ATCAACG2210900.036.0027710-11
TACATGG2193850.035.7291422-23
CATGGGG1280650.035.4097124-25
TCAACGC2086750.034.0494312-13
TGGGGTC379600.033.92334726-27
CAGAGTA2137000.033.37044518-19
AACGCAG2147450.033.134214-15
Produced by FastQC (version 0.11.5)