FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_Sambamu614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_Sambamu614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29495030
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1405540.47653452123968004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1062760.3603183315968826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322860.10946250944650676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1322800.087.589651
AGCAGTG1451550.079.6734852
GCAGTGG1606350.071.9203643
GGTATCA1713600.067.39798
TGGTATC1721800.067.128277
CAGTGGT1743550.066.321764
AGTGGTA1766550.065.573025
GTGGTAT1768800.065.4239356
GTATCAA1805300.064.02349
CAACGCA1720100.037.52112612-13
AGAGTAC1731950.037.18034418-19
GCAGAGT1744000.037.06357616-17
ACGCAGA1776250.036.35990514-15
ATCAACG1797900.035.98135810-11
TACATGG1789200.035.58960322-23
CATGGGG1057350.034.96540524-25
TCAACGC1705400.033.8686112-13
TGGGGTC315150.033.832226-27
CAGAGTA1740650.033.2424818-19
AACGCAG1752850.032.97551314-15
Produced by FastQC (version 0.11.5)