FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n02_Sambamu614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n02_Sambamu614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25148996
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1181320.46972849333627475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT908010.36105218673540684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283390.11268441889290531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1127600.087.286981
AGCAGTG1238750.079.36122
GCAGTGG1355300.072.4766543
GGTATCA1446050.067.803728
TGGTATC1457750.067.3130347
CAGTGGT1471600.066.7464
GTGGTAT1486600.066.0696646
AGTGGTA1494050.065.792265
GTATCAA1526900.064.247549
CAACGCA1446850.037.8974812-13
AGAGTAC1462250.037.3934518-19
GCAGAGT1474500.037.2150316-17
ACGCAGA1499650.036.60305414-15
ATCAACG1523950.036.07911310-11
TACATGG1507300.035.86359422-23
CATGGGG885200.035.2420324-25
TCAACGC1433100.034.18825512-13
TGGGGTC256650.033.89549326-27
CAGAGTA1469350.033.37488618-19
AACGCAG1479150.033.1168514-15
Produced by FastQC (version 0.11.5)