FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12112777
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG220060.1816759278239829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA304950.021.7414041
CGGGTAG305100.021.681922
TCGGACC237850.021.43648110
TGTTCGG239450.021.432427
CTGTTCG238750.021.407756
TTCGGAC238700.021.38929
GTTCGGA238500.021.3886938
GGTGGCT241450.021.3450621
GTGTAAC136500.021.3161131
GTGCTGC317550.021.289728
GCTGCAC317600.021.23995810
CCGGGAG56000.021.2064462
CGGACCC60550.021.15589311
AGCGCGT132700.021.1557379
GCGCGTT132800.021.1549710
CAGCGCG132750.021.13128
CGGACCT74150.021.01252611
TCCGGGA57600.020.9507311
GTCCCTT102600.020.9316357
GTAGTGC321650.020.9055295
Produced by FastQC (version 0.11.5)