FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_Sambamu614_SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_Sambamu614_SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22257693
Sequences flagged as poor quality0
Sequence length28
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA2864400.021.8713441
GGGTAGT2925600.021.841343
CGGGTAG2870750.021.8383142
TGGCTGT2280150.021.8150233
GGTGGCT2286050.021.7873521
GTGTAAC1258100.021.7354871
GTGGCTG2288550.021.717972
GGCTGTT2291400.021.7136734
GTTCGGA2299600.021.6391168
TGTTCGG2299450.021.6376557
TCGGACC2297700.021.63530310
CGTCCCT1012350.021.6330016
CTGTTCG2298900.021.618436
AGTGCTG2949300.021.605297
GCTGTTC2299450.021.5960375
TGTAACC1260350.021.592922
TCGTCCC1012000.021.5926575
TCCGGGA518900.021.5617891
GTCCCTT1015400.021.5615227
TTCGGAC2310050.021.5540839
Produced by FastQC (version 0.11.5)