FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_Sambamu614_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_Sambamu614_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27819589
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG85050.018.399631
GGAATGG76850.017.7996351
GCGGAAA47050.017.6592041
CTGGACG51300.017.504781
AGGAACG59150.017.3139468
GATGACT74150.016.9933191
ACGATAC68250.016.98677610
TATATCC91300.016.9110571
CTTGAGA83400.016.7183881
CGAGAAG92450.016.71261
TGGACGC41550.016.6255382
GTGTCCC77200.016.5858155
CCGGACA47250.016.280181
CTGGCAG71000.016.2747671
TAAGAGG61050.016.233510
GCGGAGT35950.016.2172834
GGACGCA42500.016.2030753
AGAAGAC33350.016.1952175
AGGATAC55350.016.177378
TAAGCAC94150.016.1770611
Produced by FastQC (version 0.11.5)