FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_Sambamu614_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_Sambamu614_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36286096
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG110600.018.0498431
GGAATGG101200.017.8994871
GCGGAAA60000.017.2778971
GATGACT94900.017.0351851
CTGGACG67300.016.940881
TAAGAGG80400.016.86858210
ACGATAC94500.016.85420610
AGAAGAC45700.016.7049795
GTGTCCC98100.016.6741875
AGGAACG74850.016.6363228
TATATCC118350.016.6261041
CCGGACA57000.016.4689311
AACGGGA67750.016.4547561
CGGCCCA53750.016.4543329
CTTGAGA108300.016.350041
GCGGAGT48450.016.3471264
AGGCGCT64050.016.332938
TGCGACG85400.016.3271351
CTGCCTA104500.016.312721
TAGCCGA55600.016.3025327
Produced by FastQC (version 0.11.5)