FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_Sambamu614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_Sambamu614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29495030
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG89650.018.1555161
CTGGACG53650.017.497261
GCGGAAA48950.017.4011921
GGAATGG82250.017.3805661
ACGATAC74050.016.9782610
GATGACT73250.016.931951
AGAAGAC36250.016.8113755
AGGAACG59300.016.602368
GTGTCCC80350.016.5927455
TATATCC98300.016.5579031
TAAGAGG66100.016.50753610
CTTGAGA86150.016.4787831
AGGCGCT53300.016.4693788
CCGGACA47900.016.4500891
TAAGCAC94200.016.4374311
CCAATGA74400.016.4039841
CGGCCCA43550.016.3677239
TGCGACG68250.016.3664361
CGAGAAG98650.016.3095131
TGGACGC44700.016.2907142
Produced by FastQC (version 0.11.5)