FastQCFastQC Report
Fri 1 Feb 2019
HFYGCAFXY_n01_Sambamu614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYGCAFXY_n01_Sambamu614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25148996
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG77400.018.2130621
GGAATGG69900.017.6640591
CTGGACG49100.017.3473931
ACGATAC61050.017.11629710
GCGGAAA43550.017.031251
TATATCC79350.016.6837441
AGAAGAC31350.016.5969165
GCGGAGT34050.016.573114
GATGACT63300.016.5329971
TAAGAGG57000.016.4799110
GTGTCCC69050.016.4725445
CTTGAGA73350.016.458161
TGCGACG59800.016.4517141
AACGGGA47900.016.4494861
AGGAACG48000.016.431678
TAAGCAC78800.016.4231281
CGAGAAG80600.016.3840391
ATGGGAG37850.016.3688181
TGCGGAG44850.016.3597833
GATGTCA90600.016.282263
Produced by FastQC (version 0.11.5)