FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726486
Sequences flagged as poor quality0
Sequence length41
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4454254.579505897607831No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT588330.6048741549620285No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN490180.5039641243507675No Hit
TATTTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCC264360.27179394490466546No Hit
TATTTCTAGCTCTAAAACTTGCGACGCTTAGCCTCCGTCCC233290.23985023985023984No Hit
TATTTCTAGCTCTAAAACCTGTAGGAGCGGCGTTAGTACCC107250.11026592748912607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCT347250.034.7488375
CTAGCTC342550.034.7252246
TTCTAGC350850.034.7147144
TAGCTCT345700.034.5347677
CTAACGG37000.034.5112846
AGCTCTA342700.034.4129878
CGACGTG49050.034.289224
TCTAACG39050.034.094315
GACGTAT32700.034.0185135
TGACGTA32650.033.9661144
CTAAAAC348250.033.77223612
ATATAAG323250.033.72682616
TTTCTAG362750.033.7165533
TATAAGA323150.033.7105417
GTCCCAT88950.033.70289211
TAACGGC37650.033.682167
CCCATAT310750.033.57035413
ACGTGGT49850.033.5211226
GTTACCG38050.033.424995
GCCGTTA36900.033.3600120
Produced by FastQC (version 0.11.5)