FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.cDNA.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.cDNA.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22309207
Sequences flagged as poor quality0
Sequence length41
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT5476992.455035716867928No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT735940.329881738960959No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG599850.26888001890878505No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT361260.16193314267064715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT638200.032.7589381
AGCAGTG686650.030.4100592
CAGTGGT777900.026.8874324
ATCAACG786800.026.54127511
TCAACGC788100.026.41392712
GTATCAA797300.026.3480689
CAACGCA790450.026.34623513
TGGTATC800100.026.24037
AGTGGTA803650.026.1482895
ACGCAGA796500.026.14000915
AACGCAG799250.026.07489814
GGTATCA807650.026.0217258
AGAGTAC794200.025.96579419
GTGGTAT814050.025.8042376
GAGTACT797850.025.7034720
CGCAGAG811950.025.68512716
GCAGAGT807100.025.66830417
TATCAAC817550.025.66268710
GTACTTT796950.025.63088822
CAGAGTA812500.025.54494918
Produced by FastQC (version 0.11.5)