FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.cDNA.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.cDNA.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35566556
Sequences flagged as poor quality0
Sequence length41
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT8006202.2510473041022023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1161610.3266017659961229No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT1105060.31070199768569107No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT528310.1485412306999868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT957150.032.8031921
AGCAGTG1036250.030.318052
CAGTGGT1175850.026.7369844
ATCAACG1174250.026.58235211
TCAACGC1174200.026.5033712
CAACGCA1177350.026.44684613
TGGTATC1191450.026.4250667
GTATCAA1186400.026.4148549
ACGCAGA1185250.026.27439515
AGTGGTA1205650.026.1511785
AACGCAG1191200.026.14878514
GGTATCA1205400.026.0895888
AGAGTAC1184000.026.08081219
GAGTACT1189750.025.78910820
GCAGAGT1205350.025.72640217
TATCAAC1216950.025.70586610
GTGGTAT1225750.025.6968156
GTACTTT1188600.025.67427322
CGCAGAG1217200.025.665416
CAGAGTA1216900.025.50914618
Produced by FastQC (version 0.11.5)