FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.cDNA.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.cDNA.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31663105
Sequences flagged as poor quality0
Sequence length41
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT7496332.3675283899036432No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT1028550.3248417993118489No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG911940.28801344656501626No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT493250.15578067912164648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT885650.032.755641
AGCAGTG952350.030.4713732
CAGTGGT1080150.026.8823814
ATCAACG1084850.026.53459711
TCAACGC1085550.026.44819612
CAACGCA1087400.026.39939513
TGGTATC1100050.026.3645067
GTATCAA1099150.026.3112669
ACGCAGA1095700.026.22294415
AGTGGTA1113300.026.196045
AACGCAG1099550.026.1516214
GGTATCA1110050.026.1023468
AGAGTAC1092900.026.04364619
GTGGTAT1127100.025.8338646
GCAGAGT1111850.025.73711217
TATCAAC1123400.025.71259110
GAGTACT1100200.025.70176320
CGCAGAG1121050.025.6905216
GTACTTT1098800.025.59504722
CAGAGTA1121800.025.54176118
Produced by FastQC (version 0.11.5)