FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.cDNA.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.cDNA.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29190450
Sequences flagged as poor quality0
Sequence length41
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT7102172.4330457392743177No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT964260.33033406473692595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG861030.29496975894513444No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT471240.16143636017944224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT824500.032.76851
AGCAGTG885850.030.5022772
CAGTGGT1007200.026.8515114
ATCAACG1013800.026.47386411
TCAACGC1014850.026.36924612
GTATCAA1025450.026.3097119
CAACGCA1017900.026.3071213
TGGTATC1029850.026.2791337
AGTGGTA1037500.026.2003365
ACGCAGA1024450.026.1709415
AACGCAG1028000.026.09391814
AGAGTAC1025300.025.98929219
GGTATCA1041350.025.9589378
GTGGTAT1051750.025.7851096
TATCAAC1047350.025.70791810
GCAGAGT1041900.025.65179317
CGCAGAG1049100.025.6367916
GAGTACT1032500.025.62546220
GTACTTT1030000.025.57681322
CAGAGTA1049450.025.50538618
Produced by FastQC (version 0.11.5)