FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.HashOligos.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.HashOligos.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13813881
Sequences flagged as poor quality0
Sequence length41
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434130.3142708410474942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACT2326950.034.8884432
TAACGAC2280950.034.882981
CTTACTA2339200.034.8781663
AGCTTAC2321500.034.8755341
CATGTTA1705800.034.8712582
GAGGCTG1292350.034.849081
CTGTCTA1536850.034.848872
TTACTAT2349150.034.8394934
GGCTGAG1304000.034.8260273
ACATGTT1703100.034.8232461
TGTCTAA1549050.034.8174823
GTGTGAC1391650.034.808851
TGTGACG1401600.034.7677882
TACTATC2347350.034.7650265
TCTAACG1547550.034.7567145
ACTGTCT1531350.034.7506681
CGACGTG2295150.034.74554
TGACGTA1409800.034.74414
GACGTGG2295750.034.7337725
CTAACGG1543600.034.7320376
Produced by FastQC (version 0.11.5)