FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n02_HTA.Guides.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n02_HTA.Guides.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762948
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTCTAGCTCTAAAACCGCCGTTAAGCGGAAACGATCCC16529009.860437436183659No Hit
TATTTCTAGCTCTAAAACTTGCGACGCTTAGCCTCCGTCCC14425048.605312144379377No Hit
TATTTCTAGCTCTAAAACCTGTAGGAGCGGCGTTAGTACCC6697993.9957112555619694No Hit
TATTTCTAGCTCTAAAACCTGTCAGTTGCAAACATTCGCCC5807533.464503976269568No Hit
TATTTCTAGCTCTAAAACCATGTCTCGCCCCGATATGGCCC5652453.371990416005586No Hit
TATTTCTAGCTCTAAAACGCGATATCCTATGCGGGCCCCCC5058173.017470435391197No Hit
TATTTCTAGCTCTAAAACTTCCAGCTTTACAGTGAATTCCC4531352.703193972802397No Hit
TATTTCTAGCTCTAAAACCGTTGAATGTCTCACCAAGACCC4284342.55583922350651No Hit
TATTTCTAGCTCTAAAACCTCTCTTCAGCCAGTTCTTCCCC4189952.4995305121748275No Hit
TATTTCTAGCTCTAAAACTCACACATGCTGTATCTCGACCC3148981.8785359233948589No Hit
TATTTCTAGCTCTAAAACGCATTCTCCGCGGTTGTCTACCC3147711.8777783000937545No Hit
TATTTCTAGCTCTAAAACCATTGCATCATCTTACTCTCCCC3083931.8397300999800272No Hit
TATTTCTAGCTCTAAAACCGTAGAACTCGATCAGCAGACCC2851861.7012878641632725No Hit
TATTTCTAGCTCTAAAACTTGAACGGGCCGCGGAAGCGCCC2847851.6988956835038802No Hit
TATTTCTAGCTCTAAAACTGATGAGCAAGTTCCACTATCCC2754801.6433863542379301No Hit
TATTTCTAGCTCTAAAACGTAGAGAGCGGCGCGCCTACCCC2285421.3633759407951394No Hit
TATTTCTAGCTCTAAAACCACAATTGGTCAGCTGCTTCCCC2099051.252196212742532No Hit
TATTTCTAGCTCTAAAACCGTGTAACAAGTTCTATGAGCCC2040061.217005505236907No Hit
TATTTCTAGCTCTAAAACCAGTTCCTGCAATGGTGTCGCCC1931751.1523927652820973No Hit
TATTTCTAGCTCTAAAACCACTGCTTGCTGGGAGTCCACCC1852321.1050084985051556No Hit
TATTTCTAGCTCTAAAACCGTGTGGATTAATGCGGGGACCC1536010.9163125722277489No Hit
TATTTCTAGCTCTAAAACTTGGGCTTCATGGAGTCGTCCCC1455820.8684749245777055No Hit
TATTTCTAGCTCTAAAACCTCTGCTGGGAAGTATGATGCCC1413820.8434196658010275No Hit
TATTTCTAGCTCTAAAACCAGAGAGTAGTTTCTGACTACCC1328130.7923009723587998No Hit
TATTTCTAGCTCTAAAACCGATCGACGTCCTTTCTTTACCC1307270.7798568604997164No Hit
TATTTCTAGCTCTAAAACTTGCTTAAGCTTCCGTGTCCCCC1304880.7784310969645674No Hit
TATTTCTAGCTCTAAAACCAGAGCTCTTAGTGTTCTGACCC1284640.76635684844933No Hit
TATTTCTAGCTCTAAAACCCCCACGTTCGATCTTGCACCCC1198820.715160603015651No Hit
TATTTCTAGCTCTAAAACCGCAGTTTTCCTCAGATTCACCC1163780.6942573585505366No Hit
TATTTCTAGCTCTAAAACCTGAGACCACTTTTGTATCCCCC1107620.6607548982434355No Hit
TATTTCTAGCTCTAAAACTGTCGCCCTTCTTCTTGCGTCCC1082750.6459186057249596No Hit
TATTTCTAGCTCTAAAACCTTTGGGACCGTGCCCAACGCCC1077480.6427747673022669No Hit
TATTTCTAGCTCTAAAACCTGTCGCTGGGTGTCTGTCCCCC1043830.6227007325919045No Hit
TATTTCTAGCTCTAAAACCCAGTAATTCTATTTTGTCACCC959030.5721129720142304No Hit
TATTTCTAGCTCTAAAACTTGTGAAACTTCTGTTCGACCCC938400.5598060675246382No Hit
TATTTCTAGCTCTAAAACTGTCAATACTGTGAGACTCACCC865930.5165738150592605No Hit
TATTTCTAGCTCTAAAACTGTAGAACCACTTCCGGGCACCC832210.49645802158427027No Hit
TATTTCTAGCTCTAAAACCATTCTTCTCATTGTGACAACCC814740.48603622704073296No Hit
TATTTCTAGCTCTAAAACTGGTGTGTACATGTTCCGCACCC745200.44455187715191863No Hit
TATTTCTAGCTCTAAAACCAGCTTTGGTCCATTTCAAGCCC734650.43825823476872927No Hit
TATTTCTAGCTCTAAAACCCTTTATCATCTGTGGGTGCCCC720110.42958434280175545No Hit
TATTTCTAGCTCTAAAACCTCTGCTATCTCCTGTGTCGCCC710430.42380970220751146No Hit
TATTTCTAGCTCTAAAACCTGTTAGTTGCAAACATTCGCCC660690.39413711717055977No Hit
TATTTCTAGCTCTAAGATCGGAAGAGCGTCGTGTAGGGAAA655640.3911245205795544Illumina Single End PCR Primer 1 (100% over 27bp)
TATTTCTAGCTCTAAAACCAGCTAATTCAACTTTGACCCCC634020.3782270278473691No Hit
TATTTCTAGCTCTAAAACTCGTTCTGTGATGATGCCAGCCC609980.3638858749666228No Hit
TATTTCTAGCTCTAAAACTTGTGAGCAGTGTGACCCAACCC608360.3629194578423795No Hit
TATTTCTAGCTCTAAAACTGGGGTGTTCAGGGTCCTCTCCC588560.351107692990517No Hit
TATTTCTAGCTCTAAAACCGCTTACATCAAACATCTCTCCC579580.345750640042551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG559890.3340044961065321No Hit
TATTTCTAGCTCTAAAACCTGTAGGAGCGCGTTAGTACCCC541720.3231651139167168No Hit
TATTTCTAGCTCTAAAACCTGGTTTGATGGGGTCTGTCCCC534890.3190906515966046No Hit
TATTTCTAGCTCTAAAACCGTAGGTGATGGGGCTCGGGCCC511470.3051193620597045No Hit
TATTTCTAGCTCTAAAACTGAGGTATTTCTGACCAATCCCC482820.28802809625132764No Hit
TATTTCTAGCTCTAAAACCCGCGCCGAGCGGAGGAGGACCC480840.2868469197661414No Hit
TATTTCTAGCTCTAAAACCTGGTACCTTCAAATTTGAGCCC468040.2792110313770585No Hit
TATTTCTAGCTCTAAAACGTCAACAATCTTGGCAGTTTCCC435010.2595068600105423No Hit
TATTTCTAGCTCTAAAACCTGGTTTGATGGGGTGCTGTCCC422150.2518351783946356No Hit
TATTTCTAGCTCTAAAACCTTTGACCTGGCTGACCGGACCC417070.2488046851902183No Hit
TATTTCTAGCTCTAAAACCTCAGTACAGTTCACACAAGCCC392940.2344098424692363No Hit
TATTTCTAGCTCTAAAACCCTGTTTCACTCCAAGAATCCCC391560.23358659825228834No Hit
TATTTCTAGCTCTAAAACCGGAGTACTGCAGTATGTATCCC385970.23025186261986852No Hit
TATTTCTAGCTCTAAAACCATGTAATGCTGTTGGAATGCCC366180.21844606330580996No Hit
TATTTCTAGCTCTAAAACCGTGTTGTTTTGACACCATTCCC350730.20922930739867476No Hit
TATTTCTAGCTCTAAAACTTCCAGCTTACAGTGAATTCCCC316000.18851099460548348No Hit
TATTTCTAGCTCTAAAACGATGGCTGCCGCGTAGATGTCCC310350.18514046574623985No Hit
TATTTCTAGCTCTAAAACCCTACTTGAATACTATCTGGCCC309080.18438284244513553No Hit
TATTTCTAGCTCTAAAACCTGTCAGTTGCAAACATTCCCCA308400.18397718587446552No Hit
TATTTCTAGCTCTAAAACATGTGTGCAAGCACTGTCACCCC293400.17502887916850904No Hit
TATTTCTAGCTCTAAAACTTCTGCTGATCGAGTTCTACCCC292320.17438460108568016No Hit
TATTTCTAGCTCTAAAACTCATGGAAGAGAGAGGCTCTCCC278770.16630129736129948No Hit
TATTTCTAGCTCTAAAACCCAAGAAGCGGTACTACTGTCCC277240.1653885700772919No Hit
TATTTCTAGCTCTAAAACCCCAGAATCTTTTTGGATGGCCC275930.1646070846249717No Hit
TATTTCTAGCTCTAAAACCGGTGGGGAAGACAAGGCCACCC273450.1631276312495869No Hit
TATTTCTAGCTCTAAAACCATGGATAACGACCATGTGACCC272980.16284725097280026No Hit
TATTTCTAGCTCTAAAACAGTCAGCGATAGCCGCCCGTCCC262400.15653571197619895No Hit
TATTTCTAGCTCTAAAACCACTGGCTGTGACACTCATGCCC248770.14840468394938647No Hit
TATTTCTAGCTCTAAAACTCAGCGCCTTTCTGTGATCGGCC244150.14564860548395187No Hit
TATTTCTAGCTCTAAAACGCTGCAGTACTGGCAGATGTCCC237160.14147869455897613No Hit
TATTTCTAGCTCTAAAACCGTGGAATGTGTGAAGCACCCCC226200.13494046512582394No Hit
TATTTCTAGCTCTAAAACTGTGGAGTACATGACACTGACCC220080.13128955598979367No Hit
TATTTCTAGCTCTAAAACCCATGGTCTTTGTGTCCTGGCCC217290.1296251709424858No Hit
TATTTCTAGCTCTAAAACGAAGAGCTAATATGCTCAGACCC211610.12623674546983024No Hit
TATTTCTAGCTCTAAAACCTGCTATCTGATTAAACCCCCCC204970.1222756283679935No Hit
TATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGA202120.12057545009386177No Hit
TATTTCTAGCTCTAAAACTTGAACGGGCCGCGGAAGCGCGC201320.1200982070695441No Hit
TATTTCTAGCTCTAAAACCCCGGTCCGTCAGTCCTATCCCC196760.1173779218309333No Hit
TATTTCTAGCTCTAAAACCACTGGTTACTATAAGACAGCCC189890.11327959735960523No Hit
TATTTCTAGCTCTAAAACTTGGTTTGGATCCTCCAATTCCC189860.11326170074619332No Hit
TATTTCTAGCTCTAAAACCTGGGCGAGGTGTCAAGATCCCC187390.1117882129086125No Hit
TATTTCTAGCTCTAAAACCCGATCTCTTTGACGCAGACCCC183010.1091753073504732No Hit
TATTTCTAGCTCTAAAACCTGGGCGAGGTGATCAAGATCCC180480.10766602628606854No Hit
TATTTCTAGCTCTAAAACCGATCGACTCCTTTCTTTACCCC180080.1074274047739097No Hit
TATTTCTAGCTCTAAAACCGCCGAGCGGAGGAGGAGGACCC177960.10616271075946784No Hit
TATTTCTAGCTCTAAAACTTGTCCAACAGGCGTGCCACCCC171840.1025118016234376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG454.438334E-935.0638771
ATTTCTA16225600.035.037462
CGAGCTC4450.035.0278746
GGCTCGA503.255991E-1035.021278
GGCGCTA406.226401E-835.0212678
GCTCCCT251.7055529E-435.020649
CTCGAGG301.212972E-535.0191710
CTAACGG200.002415257535.01508312
CTACAGG358.679999E-735.01508312
TAGGACG200.002415470335.01445413
ACATAGG301.2149878E-535.01068514
CGTGATT200.002417811235.0075415
AGGCTTG358.694642E-735.0075415
TTTCTAG16179800.035.0064133
CACGCGA552.3646862E-1134.99864216
AACGACT200.002420828734.9986416
GTGCATG251.7125257E-434.9963435
ACTACGC251.7125257E-434.9963435
CGCTCAC200.0024216134.99633835
CCTCGAA200.002421929734.99539634
Produced by FastQC (version 0.11.5)