FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726486
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG1883921.9368968402360318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGT558350.5740511012918746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG33200.018.9981331
GGAGCAT27800.018.9410234
TCGGAGC29750.018.2300952
GGGGGGT68600.017.75888621
CTCGAAA10550.017.4374641
GACCTGG7900.017.431791
CCGGTAG7950.017.3221531
CCTTACG7650.017.176961
GCATGCG9350.017.0895141
CGCTTCA7100.017.0269641
TACGGGC8650.016.8925971
CGTTGGG10150.016.881821
GAACCTA13250.016.819681
GTAACTG7650.016.7647131
CGATTGA8900.016.5362011
GATCGCG6700.016.47451
GGGTTGC9400.016.4394471
GACTAAC7250.016.3846871
CACCGAA6600.016.374278
TGCCAAA10750.016.3307361
Produced by FastQC (version 0.11.5)