FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.cDNA.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.cDNA.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22309207
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCATC77650.017.893391
GACTAAC53350.017.5985721
CCGGTAG66750.017.5584951
CTCGAAA71200.017.5083431
CGACCTT83450.017.417431
GACCTGG68700.017.3964181
ACTGATG58000.017.34651
TGGCTGG77550.017.3206041
CGATTGA69950.017.1606141
GAACCTA74600.017.1467881
TGCCAAA74050.017.0897711
CGGACTG45550.017.0384621
TGGACGC63550.016.9883771
CGTGAGC53400.016.9527551
GATCGCG56650.016.8514881
GGGACCT57900.016.832311
CCCTCCT119150.016.7468781
TGCGTGG87000.016.7308621
GAATGAA64250.016.7215611
ATGAATG50850.016.70465310
Produced by FastQC (version 0.11.5)