FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.cDNA.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.cDNA.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35566556
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA107450.017.7691761
ACTGATG89250.017.6272031
GGGCATC126900.017.478821
GACCTGG107450.017.4564081
TGCCAAA118000.017.4443131
TGGACGC102900.017.4219991
TGGCTGG125400.017.369651
CCGGTAG104400.017.252161
CGATTGA115350.017.2441651
GACTAAC82550.017.2131581
CGACCTT130400.017.0821651
AGCTACC79600.017.0687668
GGGAATG79700.016.9721681
GATCGCG94200.016.9462071
CGGACTG75050.016.9042551
TGCGTGG128800.016.8948251
GGCTCGA116500.016.893651
CGTTGGG121600.016.876051
GGGACCT90450.016.8592361
GAACCTA122400.016.8258111
Produced by FastQC (version 0.11.5)