FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.cDNA.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.cDNA.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31663105
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA98300.017.6927571
CCGGTAG92500.017.6553921
ACTGATG82800.017.6308441
GACCTGG100350.017.5511021
GGGCATC111250.017.5118731
CGGACTG67650.017.4341451
TGGCTGG118750.017.3168051
GACTAAC78600.017.1565971
CGATTGA100450.017.084051
CGACCTT119550.017.0340121
AGCTACC69000.017.02788
TGGACGC90000.017.0255621
CGTGAGC82800.016.9966411
TGCCAAA105500.016.9929871
GGGAATG72050.016.8942181
GAACCTA112550.016.871031
TGCGTGG116950.016.855931
TTTATGC83850.016.8088551
GGCTCGA100050.016.8059441
GATGAAA81400.016.772881
Produced by FastQC (version 0.11.5)