FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.cDNA.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.cDNA.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29190450
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG88500.017.8838391
ACTGATG72700.017.6678141
CTCGAAA89150.017.4942741
TGGCTGG108600.017.4073831
GGGCATC100200.017.3049431
CGATTGA94950.017.28841
CGACCTT107750.017.2620371
TGCCAAA101100.017.223611
GATGAAA72650.017.159551
GACCTGG85650.017.142361
GATCGCG75050.017.1145921
CGGACTG64200.017.0787561
TGGACGC85750.017.0243851
GGGAATG69050.017.0046981
ATGAATG67800.016.97317310
GTAACTG77350.016.9722921
TGCGTGG104800.016.9462321
CGTTGGG97350.016.9377021
AGCTACC63800.016.9181618
GAACCTA103050.016.897691
Produced by FastQC (version 0.11.5)