FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.HashOligos.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.HashOligos.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13813881
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATATG119150.020.4355267
GCCATAT118300.020.298936
CATATGA123250.020.232728
TTTATGC151750.019.885521
TTATGCC131100.019.807322
TATGCCA130050.019.7003673
TATGAGA126850.019.5757410
TGCCATA123500.019.5632675
ATATGAG133850.018.6068949
ATGCCAT129500.018.4540884
TATGCCC37000.018.0261141
ACTGATG32000.017.790141
AGCTTGA36000.017.6515521
CTAATGG44700.017.599661
CAATGGA41900.017.5162328
AAACCTG36250.017.4718691
TGCTACC34550.017.4195371
GAACCTA38650.017.3652651
TGGACGC41850.017.3425371
CATATAC21800.017.2908178
Produced by FastQC (version 0.11.5)