FastQCFastQC Report
Wed 30 Jan 2019
HFY7MAFXY_n01_HTA.Guides.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY7MAFXY_n01_HTA.Guides.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762948
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGCTAGAAATACTGTCTCTTATAC735990.4390576168344614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCAT642550.020.309094
TCGGAGC644550.020.2939822
CGGAGCA643300.020.290373
CTCGGAG694200.020.2369631
GCATCAG653650.020.0422657
GAGCATC649500.019.9868095
CATCAGT671200.019.8294018
GAACCTA116050.019.4036461
GACTTTT159050.019.330712
TATGTGG120350.019.31571610
AGCATCA677950.019.2797166
CTATGTG121050.019.1952889
AGGCCAC123300.019.0719321
TGACTTT194500.019.0507761
GATGCGA86600.019.0353039
ACTTTTC167800.019.029263
GTGCACG85400.019.0200548
GACCTGG41550.018.9580631
TACTTGT109750.018.9480421
ATCAGTC698050.018.8981559
Produced by FastQC (version 0.11.5)