FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7952185
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188680.2372681219061176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22500.040.27772511
ACACGTC22700.038.76578513
CACACGT23850.038.18595512
GATCGGA25450.037.6497231
ACGTCTG24050.037.4359415
CACGTCT23300.037.28626614
AGCACAC25050.036.7151110
CGTCTGA24300.036.6842116
TCGGAAG27700.036.441943
AGAGCAC26450.035.4502568
GAACTCC25700.035.05938321
ATCGGAA30950.033.0503232
CTCCAGT25500.031.91707424
CGGAAGA31800.031.8847354
TGAACTC28700.031.62612320
CTGAACT29350.030.48562419
CCAGTCA22250.030.3864526
CAGTCAC19200.030.03345727
AACTCCA32450.027.85695322
TCTGAAC42600.021.84410518
Produced by FastQC (version 0.11.5)