FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_SENS-pool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_SENS-pool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12064867
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC242670.20113773322159292TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34700.035.8567611
AGTCACG35100.034.9994228
ACACGTC37750.033.07892613
CGATCAG37600.032.91169433
CACACGT37750.032.90012412
GATCGGA37900.032.5474171
GTCACGA37650.032.50942629
CACGATC40050.030.67364931
ACGTCTG41400.030.2168915
AGCACAC41700.030.05346310
GATCTCG39200.029.5594639
CGTCTGA42450.029.46947916
CACGTCT42500.029.43480914
CAGTCAC43800.028.30432727
TCCAGTC44250.028.06733525
GTATGCC43850.027.7589545
CGTATGC44150.027.67224944
GAGCACA45200.027.4774239
CCAGTCA44950.027.32991626
GAACTCC46500.026.61246521
Produced by FastQC (version 0.11.5)