FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_S-pool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_S-pool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9463845
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16150.026.33074811
CTTAGGC16800.025.31199833
ACTTAGG16900.025.02908932
CACACGT18500.023.22926912
ACACGTC18600.023.22534613
GATCGGA18050.023.1965331
ACGTCTG19850.021.42274915
CGTCTGA20700.021.19523216
CACGTCT21150.020.53150714
AGCACAC21500.020.09262510
GAACTCC23000.019.17353221
TTAGGCA23100.018.79832834
CAGTCAC23450.018.4218127
TAGGCAT22800.017.9601735
CACTTAG24350.017.92572231
CCAGTCA24750.017.54510726
GAGCACA25500.017.2055429
TCCAGTC25700.016.80900425
AGTCACT26600.016.40944928
AGAGCAC27850.015.4305738
Produced by FastQC (version 0.11.5)