FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_RES-pool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_RES-pool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12491128
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33100.033.7153811
GATCGGA33900.031.6096611
CACACGT35850.031.50568612
GTCACAC35600.030.90531729
ACACGTC37650.029.76039513
ACGTCTG38700.029.24363715
AGCACAC39550.028.67202810
CACGTCT40050.028.20171714
CGTCTGA40350.027.82475716
GAGCACA42500.026.5759749
CAGTCAC43200.025.72868327
AGAGCAC45200.025.5859058
GAACTCC44650.025.39705821
TCCAGTC43650.025.3603525
ATCGGGG18850.024.588440
TCGGAAG48500.023.5214963
CCAGTCA47850.023.27542726
AGTCACA48250.023.22236328
CGGGGGT6950.022.98517842
TCACACT48350.022.94165830
Produced by FastQC (version 0.11.5)