FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_R-pool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_R-pool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14409242
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC402760.27951505013240807TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61850.038.08733711
CACACGT66500.035.79625712
ACACGTC66450.035.78933313
CACGTCT69050.034.4091414
GATCGGA67850.034.239551
ACGTCTG69450.034.01658215
AGCACAC70800.033.52685510
CGTCTGA71050.033.18721416
CACGATC61400.032.9431436
CGATCTC60850.032.76021638
GATCTCG61300.032.70324339
GTATGCC69850.032.5011245
GAGCACA73850.032.1726659
CATCACG72400.032.1333233
CAGTCAC73350.032.0238827
CGTATGC71600.031.6753344
AGTCACA77250.030.66927328
TCCAGTC76350.030.58876425
GAACTCC77450.030.38672421
GTCACAT78400.030.24810229
Produced by FastQC (version 0.11.5)