FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9847-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9847-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774132
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29450.035.6785511
GATCGGA29400.035.141261
ACGTCTG30750.034.0238515
ACACGTC31400.033.3911913
CACACGT32250.032.79017612
CGTCTGA32150.032.75219716
CAGTCAC32200.032.56159627
CACGTCT32450.032.24139814
AGCACAC33500.031.56666210
TCCAGTC33350.031.30385225
GAGCACA34100.031.209189
CCAGTCA34950.029.80639526
AGTCACC35450.029.57639928
GAACTCC35300.029.574621
TCGGAAG37400.028.7579233
AGAGCAC37150.028.6471338
CCAGATC36400.028.00092733
GTCTGAA39300.026.85071617
ACTCCAG38700.026.68562123
ATCGGAA40350.026.6554242
Produced by FastQC (version 0.11.5)