FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9847-3h-chipMYC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9847-3h-chipMYC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6202154
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG330820.5333953332987218TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG44900.043.0449635
GTCCCGA42900.042.63916836
ACCCGTC47350.041.1503332
CCCGTCC47700.040.9427333
CCCGATC39600.040.28343638
GCACACG49400.040.262511
CCGTCCC48650.039.9119934
TCACCCG49050.039.90760430
CACCCGT49000.039.9024131
ACGTCTG50350.039.5028315
ACACGTC50550.039.4355613
GATCGGA50200.039.3255161
TCCCGAT43750.039.0336537
CACGTCT51250.038.85302714
CACACGT51350.038.7773612
CGATCTC41200.037.9544840
CCGATCT41200.037.84525739
GTCACCC51950.037.67984829
GAACTCC53200.037.4711921
TCCAGTC53300.037.40089425
Produced by FastQC (version 0.11.5)