FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9846-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9846-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620563
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC469670.6163192929446288TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC68500.041.35328729
GCACACG69500.041.1467611
GATCGGA70150.040.2745171
TCACGCC70350.040.23383330
CACGTCT71850.040.02017214
AGTCACG71450.039.89782328
ACACGTC72350.039.8679913
CACGCCA71800.039.38997731
CGTCTGA73600.039.09917416
ACGTCTG73550.039.06457515
CACACGT73700.038.8934812
GAGCACA74200.038.601079
AGCACAC74700.038.34269710
GTATGCC65100.038.08688745
AGAGCAC76400.037.5778738
TATCTCG70750.037.49403839
TCCAGTC76700.037.2255525
CAGTCAC77150.037.0084227
GAACTCC77350.036.8836421
CCAGTCA77550.036.75950626
Produced by FastQC (version 0.11.5)