FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9846-3h-chipRed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9846-3h-chipRed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688569
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG53700.040.05500811
CACACGT56150.038.30728512
ACACGTC56400.038.25716413
GATCGGA55850.037.9322051
CACGTCT57450.037.67543414
ACGTCTG57250.037.6105515
CGTCTGA58100.037.09903716
CAGTCAC58800.036.50432227
AGCACAC59300.036.34829710
GAGCACA59950.036.1043179
TCCAGTC60200.035.50588625
CCAGTCA60600.035.3086526
AGTCACA61850.034.84969728
AGAGCAC62800.034.6091358
GAACTCC62150.034.5004721
TCGGAAG63350.034.310893
ATGTCAG62400.033.9295634
TCACATG63200.033.9272830
GTCACAT63850.033.75808729
CACATGT63700.033.62565631
Produced by FastQC (version 0.11.5)