FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9845-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9845-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601378
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCC152670.23126989546727972TruSeq Adapter, Index 5 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGCC81960.12415589593566677TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC70480.1067655874273523TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103900.042.6388211
GATCGGA103450.042.1282651
ACACGTC106650.041.70813813
CACACGT106650.041.66594312
ACGTCTG107000.041.3614315
CACGTCT107200.041.3472314
AGCACAC107850.041.1606210
GTCACAC106550.040.90262229
GAGCACA109000.040.7882849
CGTCTGA108800.040.7391816
CAGTCAC109750.039.9765327
CACACAG109100.039.96722831
AGAGCAC111600.039.8985068
TCGGAAG112750.039.8927763
TCCAGTC109500.039.8828725
GAACTCC110450.039.72316721
CCAGTCA109900.039.69676626
AGTCACA111850.039.3265428
CACAGTG111250.039.31617433
GTCTGAA113000.039.28471817
Produced by FastQC (version 0.11.5)