FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9845-3h-chipRed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9845-3h-chipRed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6844972
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG679970.9933860942017002TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTATG221040.32292316170175717TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGATG75320.11003697312421439TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG204150.043.45597511
GATCGGA202750.043.4396321
CACACGT206150.043.1216912
AGCACAC206550.043.1035410
ACACGTC206850.043.0301513
ACGTCTG206350.043.01447315
CACGTCT207100.042.94561414
GAGCACA208450.042.732249
CGTCTGA208500.042.64645816
AGAGCAC210650.042.4141248
TCGGAAG213450.042.103873
CAGTCAC209400.042.0441227
TCCAGTC209100.041.97531525
CCGTATC198750.041.94238738
CCAGTCA209550.041.8422326
GAACTCC210900.041.841121
AGTTCCG209050.041.74857734
GTTCCGT208150.041.72371335
AGTCACA211650.041.6609428
GTCACAG210700.041.6458929
Produced by FastQC (version 0.11.5)