FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9844-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9844-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6923541
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC529470.7647387370133288TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG75400.041.83613611
CACACGT77050.040.8818312
ACACGTC77000.040.8207113
GATCGGA76250.040.6511571
CGTCTGA78900.039.95177516
ACGTCTG78800.039.8597115
CACGTCT79550.039.5687614
ACTGACC78200.039.388732
GAGCACA81200.038.958689
AGCACAC81100.038.86810
GTATGCC74450.038.56212245
CAGTCAC80900.038.54691727
AGAGCAC82250.038.434268
CCAGTCA81250.038.40855826
TCCAGTC81550.038.23967725
CGTATGC75300.038.156744
TCGGAAG83800.037.859523
GAACTCC82950.037.75702721
CTCGTAT75150.037.0053442
GTCTGAA85250.036.9758917
Produced by FastQC (version 0.11.5)