FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9844-3h-chipRed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9844-3h-chipRed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6367292
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG410760.6451094122901856TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG60050.041.55196811
CACACGT61300.040.77806512
ACACGTC61650.040.65604813
GATCGGA61400.040.3677181
CACGTCT63000.039.96341314
AGCACAC63200.039.65894310
ACGTCTG63200.039.5877415
GAGCACA64000.039.1983649
CGTCTGA64450.038.88976316
CACAGTC64850.038.06007831
CAGTCAC65150.038.02296427
TCCAGTC65250.037.79228225
GTCACAG65600.037.76213529
CCAGTCA65650.037.59628726
GAACTCC66300.037.49918421
AGAGCAC67000.037.477098
TCGGAAG68450.037.2446253
CTCGTAT59950.037.0425644
AGTCACA67150.036.95749328
TCGTATG61500.036.5479845
Produced by FastQC (version 0.11.5)