FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9843-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9843-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9284967
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC95410.10275750037668416TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69000.040.23824311
ACACGTC71550.038.89851413
ACGTCTG71700.038.8485215
GATCGGA70050.038.8238331
CACGTCT72550.038.3313414
CACACGT72700.038.19035312
AGCACAC74200.037.56992710
GAGCACA76350.036.6887789
CGTCTGA75850.036.60433616
CAGTCAC75500.036.44621727
CCGATGT74600.036.37319633
GAACTCC77550.035.4247521
TCGGAAG79800.035.4152343
TCCAGTC77400.035.3189925
GTCACCG78100.035.1752829
AGAGCAC79850.035.1091848
AGTCACC78550.034.9164828
CGATGTA77500.034.89600834
CCAGTCA78850.034.72656626
ACCGATG79050.034.6671732
Produced by FastQC (version 0.11.5)