FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH9843-3h-chipRed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH9843-3h-chipRed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115609
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC183950.2585161719819063TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGCC73440.103209718240561TruSeq Adapter, Index 12 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57450.040.8476911
CACACGT58700.039.9778512
GATCGGA58300.039.617221
ACACGTC59350.039.6158313
ACGTCTG59400.039.46885315
CACGTCT59750.039.2753114
CGTCTGA60500.038.89999416
AGCACAC62550.037.58912310
GAACTCC62600.037.37939521
GAGCACA63100.037.225839
CAGTCAC62850.037.08751327
TCCAGTC63750.036.77568425
AGAGCAC64200.036.6580968
CCAGTCA63900.036.58372526
GTCACCT63650.036.5153229
AGTCACC65450.035.6485928
TCGGAAG66950.035.4577943
ACTCCAG67100.034.80551523
GTATGCC39800.034.7103145
GTCTGAA68450.034.31628417
Produced by FastQC (version 0.11.5)