FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH7797-3h-inputTop2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH7797-3h-inputTop2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7788528
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG225710.289798020884049TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45050.039.65485811
CCGTACG45650.038.8872233
ACACGTC47450.037.41204513
CGTACGT47850.037.14632434
CACACGT48050.037.0853612
GATCGGA48000.036.4923551
CGTCTGA49300.035.9168716
ACGTCTG49450.035.76242415
CACGTCT49600.035.60890614
AGCACAC50400.035.53474810
GAACTCC50850.034.82206321
GTACGTA51550.034.4801535
GAGCACA52300.034.243819
TCCAGTC51400.034.23058725
GTCACCG51250.034.15516729
CAGTCAC52350.033.60939827
ACCGTAC52700.033.4288832
TCGGAAG53800.033.3342973
AGTCACC53050.033.0386928
CCAGTCA53150.032.93419326
Produced by FastQC (version 0.11.5)