FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH7797-3h-inputMyc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH7797-3h-inputMyc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318027
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG428200.5851303910193281TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57950.040.18443711
CACGAGT57250.040.1648731
AGTCACG58500.039.4604928
ACGTCTG59400.039.16562715
CGAGTGG58700.039.05773533
ACGAGTG59250.038.88504432
GATCGGA59100.038.7426571
TCACGAG59550.038.65136730
ACACGTC60400.038.59168613
GTCACGA59800.038.4145329
CACACGT61150.038.1919512
CACGTCT60900.038.1640114
AGCACAC61800.037.82666410
CGTCTGA62050.037.5654816
GAGCACA63750.036.8460779
CAGTCAC63550.036.46637727
TATCTCG59100.036.09041241
TCCAGTC64200.036.0270825
GAACTCC64750.035.8600521
AGAGCAC65750.035.725538
Produced by FastQC (version 0.11.5)