FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH7797-3h-chipTop2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH7797-3h-chipTop2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251697
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG192830.2659101724741119TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36250.035.93715711
ACGTCTG36550.035.334415
GATCGGA36600.034.6902851
CGTCTGA37650.034.18253316
CACACGT38500.033.89537412
ACACGTC38700.033.77834713
GAACTCC39100.033.2026121
CAGTCAC40100.032.82348627
CACGTCT39700.032.5307914
GAGCACA40600.032.308429
AGAGCAC41750.031.7420568
TCCAGTC40950.031.70261425
AGCACAC41450.031.70016510
TCGGAAG42050.031.1967013
ACTCCAG41900.030.98382223
CCAGTCA41850.030.91331926
TATCTCG33200.030.36079441
GTCTGAA42800.030.33229317
GTCACAT43400.030.0166429
AGTCACA43700.029.9650328
Produced by FastQC (version 0.11.5)