FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH7797-0h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH7797-0h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7890479
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG113860.1443004917698913TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG25550.034.43176731
AGTCACG25800.033.6620928
GCACACG26250.033.51358811
ACACGTC28400.031.45180913
ACGTCTG28700.031.2014415
ACGTGGC28050.031.12234532
GATCGGA27900.030.5799641
CACACGT29050.030.43826512
CGTGGCC29650.029.67054633
TCACGTG29950.029.52359230
AGCACAC30350.029.28275110
CGTCTGA30550.029.16469616
GTCACGT29900.029.12146829
CACGTCT30700.029.02219614
GGCCTTA30350.028.76381536
GAACTCC30850.028.58935721
CAGTCAC31500.027.9279927
GAGCACA32500.027.4148149
TCCAGTC32350.027.19417825
TATCTCG24800.026.21921241
Produced by FastQC (version 0.11.5)