FastQCFastQC Report
Wed 30 Jan 2019
HFY77AFXY_n01_AH7797-0h-chipTop2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFY77AFXY_n01_AH7797-0h-chipTop2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6995232
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20550.031.53232811
AGTCACG20950.030.60808628
ACGTCTG21350.029.82386215
ACACGTC22300.029.25960513
GATCGGA21900.028.779611
CACGTCT22850.028.4568614
GTCACGT22550.028.13700529
CACACGT23900.027.67736412
CGTCTGA23450.027.34496516
GAACTCC24300.026.66622521
ATCGGGG12700.026.57436442
TCGGGGG6550.026.1064443
CGTTTCG23800.025.90293333
AGCACAC25250.025.84115610
TCCAGTC25100.025.72666725
GTTTCGG24200.025.56775934
CCAGTCA25800.024.67982526
CAGTCAC26200.024.56066527
GAATCGG15650.024.15295640
GAGCACA27450.023.77019
Produced by FastQC (version 0.11.5)