FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18164602
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14773688.133225269675604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACTG828250.066.627096
CCACTAG551600.066.251867
CCACTGT719100.066.186437
CTCCACT421200.065.757155
ATCCACT668000.065.702995
TCCACTA615800.065.4868856
CACTAGT503750.065.3885968
CCACTCT301850.064.939277
GTCCACT345900.064.747475
TCCACTC350900.064.6964656
CTAGTGG520000.064.0563810
CACTGTA673050.064.0444958
TTCCACT461350.063.5534365
ACTAGTG514750.063.5464329
ACTGTAG740200.060.8997889
TATCCAC220000.059.36674
AATCCAC300550.059.3458984
CGTCCAC102600.059.0619934
ACTCCAC174850.059.02884
ATTCCAC206300.058.9336244
Produced by FastQC (version 0.11.5)