FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_Ler_Lib10_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_Ler_Lib10_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345672
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25650.7420329098104561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAA207.916977E-570.006864
CCGACGT207.916977E-570.006834
TTAGTCT150.002222727870.006861
TACGTCT207.916977E-570.006867
TCGTTCC455.456968E-1269.996682
AGTCCCC455.456968E-1269.996684
CCGATCC455.456968E-1269.996682
TCATTCC900.069.996682
TCCTCCA1800.069.996683
ACCTATC455.456968E-1269.996681
TCTCCAC12600.069.996684
CAGCTCC455.456968E-1269.996682
CTGATTC455.456968E-1269.996681
CATCATC455.456968E-1269.996681
CGCCACT455.456968E-1269.996685
CCATTCC900.069.996682
TCAGTCC455.456968E-1269.996682
TATTTTC252.8375907E-669.996671
CCTTCCA2300.069.996673
ATCCTTC353.7052814E-969.996671
Produced by FastQC (version 0.11.5)