FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_Col_Lib09_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_Col_Lib09_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654516
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28970.4426171399935219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACG950.070.0074161
TCATTCG750.070.0074161
CCTACCG550.070.0074161
CACCTCG252.83784E-670.0074161
AGGCCCG252.83784E-670.0074161
TGACCCG252.83784E-670.0074161
AGGCACG455.456968E-1270.0074161
TACTGCG455.456968E-1270.0074161
ACCAGCG500.070.0074161
CTGCACG455.456968E-1270.0074161
TGCTACG455.456968E-1270.0074161
TTTGACG455.456968E-1270.0074161
TTGCACG500.070.0074161
CACGTCG650.070.0074161
TCGTTCG301.0228541E-770.007411
AATCCCG1050.070.007411
CCCCTCG401.3460522E-1070.007411
CACGGCG150.00222354170.007411
CCTAGCG301.0228541E-770.007411
AGCCCCG301.0228541E-770.007411
Produced by FastQC (version 0.11.5)