FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_UB_f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_UB_f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916525
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860799.391887837211204No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC29810.3252502659501923Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC15200.066.3147661
GCCGTAT16450.065.5308960
CGTATCA16700.065.1786362
CGCCGTA14900.064.59630659
TCGCCGT14650.063.79088258
GGTCGCC17600.063.43997656
GTCGCCG17550.063.22183257
TGGTCGC16250.062.03318455
GGTGGTC18000.061.6412953
GTGGTCG17650.060.28563354
TCGGTGG16300.059.477651
CTCGGTG12950.059.18826750
CGCCCTA1550.058.70876359
CGGTGGT17950.058.69630452
GGTAGCC3350.058.50974756
GCCGGAT2600.056.5375860
TCTCGGT12700.056.49518249
GCCGGAC252.3909647E-455.99912623
GCCCTAT2000.055.99912660
GGTCCCC1400.055.00214456
Produced by FastQC (version 0.11.5)